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peroxidase avidin biotin complex method  (Vector Laboratories)


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    Structured Review

    Vector Laboratories peroxidase avidin biotin complex method
    Peroxidase Avidin Biotin Complex Method, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peroxidase+avidin+biotin+complex+method/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pm40674933-64-1-6
    Average 99 stars, based on 12758 article reviews
    peroxidase avidin biotin complex method - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: γ-H2AX: A useful tool to detect DNA damage in sudden cardiac death heart tissues, an experimental study.
    Article Snippet: After washing, sections were incubated for 1 h at RT with the secondary anti-rabbit biotinylated antibody (BA-1000, Vector Laboratories) diluted 1:200 in PBS. .. The peroxidase avidin–biotin complex method (PK-6100, Vector Laboratories) was applied for 1 h at RT using 3′,3′ diaminobenzidine hydrochloride (DAB; D7304, Sigma) as chromogen. ..

    Article Title: Placental expression of substance P and vasoactive intestinal peptide: evidence for a local effect on hormone release.
    Article Snippet: Afterward the sections were incubated overnight with one of the following primary antibodies diluted in PBS containing 1% normal serum: 1) a rabbit polyclonal antibody anti-SP (Peninsula Laboratories, Belmont, CA), dilution 1:200 vol/vol; 2) a rabbit polyclonal antibody anti-VIP (Peninsula), dilution 1:100 vol/vol; 3) a mouse monoclonal antibody antihuman pan-cytockeratin (Dako, Glostrup, Denmark), dilution 1:500 vol/vol; and 4) a mouse monoclonal antibody anticytokeratin 7 (Dako), dilution 1:200 vol/vol. .. After washing with PBS, the bound antibody was visualized by the peroxidase avidin biotin complex method (10) using the following biotinylated secondary antibodies: for the primary polyclonal antibodies, a goat antirabbit (Vector Laboratories, Burlingame, CA), dilution 1:200 vol/vol; for the primary monoclonal antibodies, a horse antimouse (Vector Laboratories) dilution 1:200 vol/vol. .. 3 ,3 -Diaminobenzidine hydrochloride (Sigma, St. Louis, MO) was used as chromogen.

    Article Title: Role of Activin-A and Myostatin and Their Signaling Pathway in Human Myometrial and Leiomyoma Cell Function
    Article Snippet: After washing with PBS, cells were incubated with biotinylated antimouse IgG made in horse (for PCNA and fibronectin) or biotinylated antirabbit IgG made in goat (for Ki-67) diluted 1:200 (Vector Laboratories). .. The peroxidase avidin biotin complex method (Vector Laboratories) was performed for 1 hour at room temperature using 3′, 3′ diaminobenzidine tetrahydrochloride (Sigma-Aldrich) as chromogen. ..

    Bioprocessing:

    Article Title: Placental expression of substance P and vasoactive intestinal peptide: evidence for a local effect on hormone release.
    Article Snippet: Afterward the sections were incubated overnight with one of the following primary antibodies diluted in PBS containing 1% normal serum: 1) a rabbit polyclonal antibody anti-SP (Peninsula Laboratories, Belmont, CA), dilution 1:200 vol/vol; 2) a rabbit polyclonal antibody anti-VIP (Peninsula), dilution 1:100 vol/vol; 3) a mouse monoclonal antibody antihuman pan-cytockeratin (Dako, Glostrup, Denmark), dilution 1:500 vol/vol; and 4) a mouse monoclonal antibody anticytokeratin 7 (Dako), dilution 1:200 vol/vol. .. After washing with PBS, the bound antibody was visualized by the peroxidase avidin biotin complex method (10) using the following biotinylated secondary antibodies: for the primary polyclonal antibodies, a goat antirabbit (Vector Laboratories, Burlingame, CA), dilution 1:200 vol/vol; for the primary monoclonal antibodies, a horse antimouse (Vector Laboratories) dilution 1:200 vol/vol. .. 3 ,3 -Diaminobenzidine hydrochloride (Sigma, St. Louis, MO) was used as chromogen.



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